NCERT Solutions for Class 12 Biology Chapter 11

Biotechnology : Principles and Processes Class 12

Chapter 11 Biotechnology : Principles and Processes Exercise Solutions

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Exercise : Solutions of Questions on Page Number : 205

Q1 :  

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Answer :

Recombinant proteins are obtained from the recombinant DNA technology. This technology involves the transfer of specific genes from an organism into another organism using vectors and restriction enzymes as molecular tools.

Ten recombinant proteins used in medical practice are -

Recombinant protein

Therapeutic use

1.

Insulin

Treatment for type I diabetes mellitus

2.

Interferon-α

Used for chronic hepatitis C

3.

Interferon -β

Used for herpes and viral enteritis

4.

Coagulation factor VII

Treatment of haemophilia A

5.

Coagulation factor IX

Treatment of haemophilia B

6.

DNAase I

Treatment of cystic fibrosis

7.

Anti-thrombin III

Prevention of blood clot

8.

Interferon B.

For treatment of multiple sclerosis

9.

Human recombinant growth hormone

For promoting growth in an individual

10.

Tissue plasminogen activator

Treatment of acute myocardial infection

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Q2 :  

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.


Answer :

The name of the restriction enzyme is Bam H 1.

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Q3 :  

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?


Answer :

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Q4 :  

What would be the molar concentration of human DNA in a human cell? Consult your teacher.


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Q5 :  

Do eukaryotic cells have restriction endonucleases? Justify your answer.


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Q6 :  

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


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Q7 :  

Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.


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Q8 :  

Can you recall meiosis and indicate at what stage a recombinant DNA is made?


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Q9 :  

Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?


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Q10 :  

Describe briefly the followings:

(a) Origin of replication

(b) Bioreactors

(c) Downstream processing


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Q11 :  

Explain briefly

(a) PCR

(b) Restriction enzymes and DNA

(c) Chitinase


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Q12 :  

Discuss with your teacher and find out how to distinguish between

(a) Plasmid DNA and chromosomal DNA

(b) RNA and DNA

(c) Exonuclease and Endonuclease


Answer :

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<< Previous Chapter 10 : Microbes in Human Welfare Next Chapter 12 : Biotechnology and its Applications >>

Biology : CBSE NCERT Exercise Solutions for Class 12th for Biotechnology : Principles and Processes will be available online in PDF book form soon. The solutions are absolutely Free. Soon you will be able to download the solutions.

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